RT-PCR and Dot Blot hybridization methods for a universal detection of tospoviruses
نویسندگان
چکیده
منابع مشابه
Rt-pcr and Dot Blot Hybridization Methods for a Universal Detection of Tospoviruses
Transcriptase reverse polymerase chain reaction (RTPCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species tested were Tomato spotted wilt virus, Tomato chlorotic spot virus, Groundnut ringspot virus, Chrysanthemum stem necrosis virus, Impatiens necrotic spot virus, Zucchini lethal chlorosis virus, Iris ye...
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Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
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ژورنال
عنوان ژورنال: Fitopatologia Brasileira
سال: 2001
ISSN: 0100-4158
DOI: 10.1590/s0100-41582001000200009